Ukulima i-Shake-flask culturing kuhlukile ekukhulisweni kwendawo lapho amangqamuzana evezwa ngokuqondile ekugxilweni kwe-oxygen ephezulu. Emasikweni e-shake-flask, amaseli achayeka ekugxilweni komoyampilo ophansi njengoba ama-microorganisms amisiwe emhluzweni wamasiko.