Izinaliti zokujova i-chromatograph yegesingokuvamile sebenzisa u-1ul no-10ul. Nakuba inaliti yomjovo incane, ibalulekile. Inalithi yomjovo ishaneli exhuma isampula kanye nethuluzi lokuhlaziya. Ngenaliti yomjovo, isampula lingangena kukholamu yechromatographic futhi lidlule kumtshina ukuze kuhlaziywe i-spectrum eqhubekayo. Ngakho-ke, ukugcinwa nokuhlanzwa kwenaliti yomjovo kuwukugxila kokunaka kwansuku zonke kwabahlaziyi. Uma kungenjalo, ngeke nje kuthinte ukusebenza kahle komsebenzi, kodwa futhi kubangele ukulimala kwensimbi. Umfanekiso olandelayo ubonisa izingxenye zenaliti yomjovo.
Ukuhlukaniswa kwezinaliti zomjovo
Ngokubukeka kwenaliti yomjovo, ingahlukaniswa izinaliti zomjovo wenaliti eyi-conical, izinaliti zomjovo wenaliti ye-bevel, nezinaliti ze-flat-head injection. Izinaliti ze-Conical zisetshenziselwa umjovo we-septum, onganciphisa umonakalo ku-septum futhi umelane nemijovo eminingi. Asetshenziswa kakhulu kuma-injection azenzakalelayo; Izinaliti ze-bevel zingasetshenziswa ku-septa yomjovo, okulula ukuyisebenzisa. Phakathi kwazo, izinaliti ze-26s-22 zifaneleka kakhulu ukusetshenziswa ku-septa yomjovo ku-chromatography yegesi; izinaliti zomjovo wekhanda eliyisicaba zisetshenziswa kakhulu kumavalvu omjovo kanye namasampula wamapayipi amakhromatograph ewuketshezi asebenza kahle kakhulu.
Ngokwendlela yomjovo, ingahlukaniswa ngenaliti yomjovo othomathikhi kanye nenaliti yomjovo owenziwe ngesandla.
Ngokwezidingo ezihlukene zokuhlaziya inaliti yomjovo ku-chromatograph yegesi kanye noketshezi lwe-chromatograph ewuketshezi, ingahlukaniswa ibe inaliti yomjovo wegesi kanye nenaliti yomjovo we-liquid. Inalithi yomjovo wegesi i-chromatography ngokuvamile idinga umjovo omncane, futhi umthamo womjovo ovame kakhulu ngu-0.2-1ul, ngakho inaliti yomjovo ehambisanayo ngokuvamile iba ngu-10-25ul. Inaliti ekhethiwe iyinaliti yohlobo lwe-cone, elungele ukusebenza komjovo; uma kuqhathaniswa, umthamo womjovo we-chromatography owuketshezi uvame ukuba mkhulu, futhi umthamo womjovo ojwayelekile ungu-0.5-20ul, ngakho-ke ivolumu yenaliti ehlobene nayo inkulu, ngokuvamile i-25-100UL, futhi ithiphu yenaliti iphansi ukuvimbela ukunwaya i-stator.
Ekuhlaziyweni kwe-chromatographic, inaliti yomjovo esetshenziswa kakhulu iyinaliti yomjovo omncane, elungele ngokukhethekile i-chromatograph yegesi kanye nokuhlaziywa koketshezi lwe-chromatograph. Iphutha layo lomthamo liphelele ngu-±5%. Ukusebenza kwe-airtight imelana ne-0.2Mpa. Ihlukaniswe yaba izinhlobo ezimbili: i-liquid storage injector kanye ne-liquid storage injector. Uhla lokucaciswayo lomjovo omncane ongewona uketshezi ngu-0.5μL-5μL, futhi ububanzi bokucaciswa komjovo omncane owuketshezi ngu-10μL-100μL. Inaliti yomjovo emincane iyithuluzi lokunemba elibalulekile.
Ukusetshenziswa kwe-injector
(1) Hlola umjovo ngaphambi kokuwusebenzisa, hlola ukuthi isipetu sinokuqhekeka yini nokuthi ichopho lonaliti libhobokile yini.
(2) Susa isampula esele kumjovo, ugeze i-injector nge-solvent izikhathi ezingu-5 ~ 20, futhi ulahle uketshezi olungcolile kusukela izikhathi ezingu-2 ~ 3 zokuqala.
(3) Susa amagwebu kumjovo, cwilisa inaliti ku-solvent, bese udweba isampula ngokuphindaphindiwe. Lapho ukhipha isampula, amabhamuza kumjovo angashintsha ngokushintsha okuma mpo kweshubhu.
(4) Uma usebenzisa umjovo, qala ugcwalise umjovo ngoketshezi, bese ukhipha uketshezi kumthamo odingekayo womjovo.
Ukugcinwa kwenaliti yomjovo
(1) Amasampula e-viscosity aphakathi nendawo ukuya phezulu kufanele ahlanjululwe noma kukhethwe inaliti yomjovo omkhulu ophakathi nendawo ngaphambi kokusetshenziswa.
(2) Lapho kuhlanzwa inaliti, kufanele kusetshenziswe amathuluzi okuhlanza, njengocingo oluqondisayo noma i-stylet, ama-tweezers, nezinto ezifakwa emanzini kufanele kusetshenziswe ukuhlanza udonga yenaliti.
(3) Ukuhlanza okushisayo: Ukuhlanza okushisayo kusetshenziselwa ukususa izinsalela eziphilayo enalitini, ikakhulukazi ukuhlaziya umkhondo, indawo yokubilisa ephezulu kanye nezinto ezinamathelayo. Ngemuva kwemizuzu embalwa yokuhlanza okushisayo, ithuluzi lokuhlanza inaliti lingasetshenziswa futhi.
Ukuhlanza inaliti yomjovo
1. Udonga lwangaphakathi lomjovo lungahlanzwa nge-solvent ephilayo. Lapho uhlanza, sicela uhlole ukuthi induku yokuphusha inaliti yomjovo ingahamba kahle yini;
2. Uma inaliti yokusunduza induku inganyakazi kahle, induku yokuphusha ingasuswa. Kunconywa ukuthi uyisule ngendwangu ethambile efakwe ku-solvent ye-organic.
3. Sebenzisa ngokuphindaphindiwe i-solvent ephilayo ukuze ufise. Uma ukumelana nenduku yokusunduza inaliti yomjovo kukhula ngokushesha ngemva kwezifiso ezimbalwa, kusho ukuthi kusekhona ukungcola okuncane. Kulokhu, inqubo yokuhlanza idinga ukuphindaphindiwe.
4. Uma inaliti yomjovo iphusha induku ingahamba kahle futhi inganyakazi, hlola ukuthi inaliti ivinjiwe yini. Hlambulula inaliti ngokuphindaphindiwe nge-solvent ephilayo futhi uhlole ukuma kwesampula ephushwa ngaphandle yenaliti.
5. Uma inaliti yomjovo ijwayelekile, isampula izogeleza iphume ngomugqa oqondile. Uma inaliti ivalekile, isampula izofafazwa ngenkungu enhle ukusuka ohlangothini olulodwa noma i-engeli. Ngisho noma i-solvent ngezinye izikhathi igeleza ngomugqa oqondile, qaphela ukuthi ukugeleza kungcono kunokujwayelekile (vele uqhathanise ukugeleza nenaliti yomjovo entsha, engavinjiwe).
6. Ukuvinjelwa kwenaliti kuzobhubhisa ukukhiqizwa kabusha kokuhlaziywa. Ngenxa yalesi sizathu, ukunakekelwa kwenaliti kuyadingeka. Sebenzisa into efana nocingo ukuze ususe ukuvaleka enalitini. Inalithi ingasetshenziswa kuphela uma isampula iphuma ngokujwayelekile. Ukusebenzisa i-pipette ukuze ufune uketshezi noma isicoci sesirinji nakho kungasusa ngempumelelo ukungcola enalitini.
Izinyathelo zokuqapha lapho usebenzisa inaliti yomjovo
Ungayibambi inaliti yesirinji nengxenye yesampula ngezandla zakho, futhi ungabi namabhamuza (uma ufisa, khipha kancane, ngokushesha, bese ufisa kancane, phinda izikhathi eziningana, 10 Umthamo wenaliti yensimbi yesirinji ye-μl ingu-0.6 μl. Uma kukhona amabhamuza, awukwazi ukuwabona Thatha u-1-2μl ngaphezulu bese ukhomba ithiphu yenaliti phezulu kuze kube amabhamuza aya phezulu, bese uphusha induku yenaliti ukuze ukhiphe amabhamuza. isipetu esinomgogodla sizwakala siyisicaba) Isivinini somjovo kufanele sisheshe (kodwa singasheshi kakhulu), gcina isivinini esifanayo somjovo ngamunye, bese uqala ukujova isampula uma ithiphu yenaliti ifika maphakathi negumbi lokuhwamuka.
Ungavimbela kanjani inaliti yomjovo ukuthi ingagobi? Abaningi abaqalayo abenza ukuhlaziya i-chromatography bavame ukugoba inaliti kanye nenduku yesirinji yesirinji. Izizathu yilezi:
1. Imbobo yomjovo isongwe kakhulu. Uma igoqwe ngokuqinile kakhulu ezingeni lokushisa legumbi, isivalo se-silicone sizonwebeka futhi siqine lapho izinga lokushisa legumbi lokuhwamuka likhuphuka. Ngalesi sikhathi, kunzima ukufaka isirinji.
2. Inaliti ibhajwe engxenyeni yensimbi yembobo yomjovo lapho indawo ingatholakali kahle.
3. Induku yesirinji igobile ngoba kusetshenziswa amandla amaningi ngesikhathi sokujova. Ama-chromatograph amangalisayo, angenisiwe afika ne-injector rack, futhi ukujova nge-injector rack ngeke kugobe induku yesirinji.
4. Ngenxa yokuthi udonga lwangaphakathi lwesirinji lungcolile, induku yenaliti iyaphushwa futhi igotshwe ngesikhathi sokujova. Ngemva kokusebenzisa isirinji isikhathi esithile, uzothola into encane emnyama eduze nangaphezulu kweshubhu yenaliti, futhi kuyoba nzima ukumunca isampula bese ujova. Indlela yokuhlanza: Khipha induku yenaliti, jova amanzi amancane, ufake induku yenaliti endaweni engcolile bese uphusha futhi uyidonse ngokuphindaphindiwe. Uma kungasebenzi kanye, phinda ujove amanzi kuze kukhishwe ukungcola. Ngalesi sikhathi, uzobona ukuthi amanzi kusirinji aba nemfucumfucu. Khipha induku yenaliti bese uyisula ngephepha lokuhlunga, bese uyigeza ngotshwala izikhathi eziningana. Uma isampula okumele lihlaziywe liyisampula eliqinile elincibilikisiwe ku-solvent, geza isirinji nge-solvent ngesikhathi ngemva kokujova.
5. Qiniseka ukuthi awunyakazi lapho ujova. Uma ulangazelela ukusheshisa, isirinji izogotshwa. Uma nje unekhono lokujova, kuzoshesha.
Isikhathi sokuthumela: Jun-19-2024