OkuhlukileI-HPLC Sampling VialHlola
Ingabe izinto zingcono uma ukhokha inani elithe xaxa?
Uyini umehluko wamabhodlela e-HPLC emikhiqizo ehlukene
Njengenkampani yokuhlola i-mass spectrometry, ukukhetha kwezinto ezisetshenziswayo okuhlola bekulokhu kusekelwe kudatha.Ocwaningweni olungenalutho, kuzohlale kuneziqongo zezipoki ezingachazeki, kusukela kusincibilikisi?Kusukela ku-vial?Noma imikhiqizo yepulasitiki kusukela inqubo isizinda?Sonke sizohlola ngamunye ngamunye ngomoya wokuphula i-casserole futhi sicele kuze kube sekupheleni.Ukuze siqinisekise ikhwalithi yokuhlola, senze uchungechunge lokuhlolaizitsha zesampula, ukuze nje uthole ethembeke kakhulu.Bheka imiphumela yethu yokuhlola, mhlawumbe wena, njengontanga, ubuye waphefumulelwa?
Izinhlobo eziyi-11 zamabhodlela omjovo kanye nohlobo olungu-1 lweshubhu lokutholisa elibamba iqhaza kulokhu kuhlola.Ake sibone ukuthi amabhodlela omjovo abukeka kanjani~~~
isifinyezo
1. Impahla yebhodlela lomjovo ifana ngokuqhathaniswa, ukubukeka ku-symmetrical, kanye nokuvumelana kwe-batch kuhle.
2. Impahla yesampula yebhodlela/ishubhu yangaphakathi iyipulasitiki nengilazi, futhi intengo yengilazi ibiza kakhulu.
3. Intengo yeyunithi yebhodlela lomjovo elihlanganisiwe leshubhu lepulasitiki licishe libe yiyuan engu-1, futhi umehluko wentengo awumkhulu.
4. I-J yepulasitiki yohlobo D lweshubhu yangaphakathi idinga ukusetshenziswa nebhodlela lomjovo elithi flat -bottom , elibiza kakhulu kunebhodlela lomjovo leshubhu lepulasitiki elihlanganisiwe elihlanganisiwe.
5. Intengo yeyunithi yengilazi yebhodlela elihlanganisiwe lomjovo weshubhu yangaphakathi iphakathi kwamayuan angu-3 no-7 yuan, futhi igebe lentengo likhulu, futhi intengo yohlobo lokuphepha B iphezulu kakhulu.
6. Intengo yezitsha zokujova ingilazi (iphansi eliyisicaba, alikho ishubhu elingaphakathi) lingu-0.6-2 yuan, futhi intengo ka-A iphakeme kakhulu.
Bheka imiphumela yethu yokuhlolwa elandelayo ~~ Izimo zokuhlola
Imishini yokuhlola: LC /MS (1290-6470) Isigaba sikamakhalekhukhwini: A: amanzi ahlanzekile B: i-acetonitrile Izinga lokugeleza: 0.3ml/min
Indlela: ukuskena okugcwele ngemodi ephozithivu kanye nemodi engalungile Izinyathelo zokuhlola:
Kala ngokunembile umthamo othile wamanzi ahlanzekile futhi
i-acetonitrile (ivolumu yesampula yebhodlela lomjovo elihlanganisiwe neshubhu lokufaka kanye neshubhu elifakayo lingu-100 μL, kanye nevolumu yesampula yebhodlela lomjovo ngaphandle kweshubhu lokufaka ngu-500 μL.) ohlotsheni ngalunye lwebhodlela lomjovo, i-vortex engu-1min, sebenzisa umkhiqizo ofanayo kanye nekepisi yemodeli (i-Agilent 5190-9024), bese ujova isampula emshinini.Yenza ukuskena okugcwele ngezindlela ze-ion eziphozithivu nezingezinhle, ngokulandelana.
Imodi ye-ion ye-vial yomjovo + i-acetonitrile positive
Qaphela: Uma ubancane inombolo yeziqongo nokuphakama kokuphakama, kuba ngcono.Idatha engenhla ingeyereferensi kuphela, futhi inesibopho samasampuli okuhlola kuphela.
Ibhodlela lomjovo + imodi ye-ion ephozithivu yamanzi
Qaphela: Uma ubancane inombolo yeziqongo nokuphakama kokuphakama, kuba ngcono.Idatha engenhla ingeyereferensi kuphela, futhi inesibopho samasampuli okuhlola kuphela.
Isampula ye-vial + imodi ye-acetonitrile negative ion
Qaphela: Uma ubancane inombolo yeziqongo nokuphakama kokuphakama, kuba ngcono.I
idatha engenhla ingeyereferensi kuphela, futhi inesibopho samasampuli okuhlola kuphela.
Ibhodlela lomjovo + imodi ye-ion eyinegethivu yamanzi
Qaphela: Uma ubancane inombolo yeziqongo nokuphakama kokuphakama, kuba ngcono.I
idatha engenhla ingeyereferensi kuphela, futhi inesibopho samasampuli okuhlola kuphela.
Ukuhlelwa kwefomu:
isifinyezo
ukusebenza kwamakhemikhali kwesitsha somjovo weSpectrum J kanye nesitsha somjovo esingu-A kungcono, kodwa lesi sakamuva asiphindaphindeki kahle.Imodeli ye-B nayo yenze kahle kakhulu, kodwa intengo yayisohlangothini oluphezulu.Kubonakala sengathi izinto ezibizayo zenza umqondo omncane, kodwa ngihlale nginomuzwa wokuthi i-premium yomkhiqizo ingaphezulu kancane.Kazi nawe uzizwa ngendlela efanayo ~~
Fingqa
izinhlobo ezahlukene zamabhodlela omjovo .Lezi zimpawu zizodala imiphumela engaziwa ekuhlonzeni kwethu okuhlosiwe, kodwa kunzima ukuthola umjovo "ohlanzekile" ngokuphelele emakethe.ibhodlela.izitsha zesampula kanye nezinto ezifakiwe ezibamba iqhaza ekuhlolweni, i-J yesampula ye-vial kufanelekile ukukhetha, kodwa ayiwafanele amasampula amancane (cishe u-<500μL).Imodeli ye-B nayo inhle.Idizayini edidiyelwe yethubhu ye-intubation yangaphakathi inezinhlobonhlobo zezinhlelo zokusebenza, kodwa iyabiza.
Isikhathi sokuthumela: Jun-17-2022