Sicela uzikhethele ngokwakho ngokusekelwe esimweni selabhorethri yakho.
Kubalulekile ukuthola indlela ephumelelayo yokuhlanza izitsha zesampula
Njengamanje, kunenani elikhulu lamasampula emikhiqizo yezolimo (eminye imikhiqizo yamakhemikhali, ama-organic acid, njll.) adinga ukuhlolwa nge-chromatography ewuketshezi kanye ne-gas chromatography njalo ngonyaka.Ngenxa yenani elikhulu lamasampula, kunenombolo enkulu yezitsha zesampula ezidinga ukuhlanzwa ngesikhathi sokutholwa, okungachithi nje kuphela isikhathi futhi kunciphise ukusebenza kahle komsebenzi, kodwa futhi ngezinye izikhathi kubangela ukuphambuka emiphumeleni yokuhlola ngenxa yokuhlanzeka izitsha zesampula ezihlanziwe.
Izitsha zesampula ze-chromatographic zenziwa ikakhulukazi ngengilazi, akuvamile ukuthi zenziwe ngepulasitiki.Izitsha zesampula ezilahlwayo ziyabiza, zimosha, futhi zingcolisa imvelo.Amalabhorethri amaningi ahlanza izitsha zesampula futhi aphinde azisebenzise.
Njengamanje, izindlela ezisetshenziswa kakhulu zaselabhorethri zokugeza izitsha zifakwa ikakhulukazi okokuhlanza, okokuhlanza, izinyibilikisi eziphilayo kanye nokugeza nge-asidi, bese kuba khona amashubhu amancane okuxubha okuhleliwe.
Le ndlela evamile yokukhuhla inezinkinga eziningi:
Ukusetshenziswa kwe-detergent kudla amanzi kakhulu, isikhathi sokugeza side, futhi kukhona amakhona okunzima ukuwahlanza.Uma kuyisampula yepulasitiki, kulula ukuba nezimpawu zebhulashi ngaphakathi kodonga lwebhodlela, okuthatha izinsiza eziningi zokusebenza.Ku-glassware engcoliswe kakhulu yizinsalela ze-lipid namaprotheni, isisombululo se-alkaline lysis sisetshenziselwa ukuhlanza, futhi imiphumela emihle ifinyelelwa.
Lapho uhlaziya amasampula nge-LC/MS/MS, ukuhlanzwa kwezitsha zomjovo kubaluleke kakhulu.Ngokwendlela yokuhlanza ye-glassware, indlela yokuhlanza ikhethwa ngokuya ngezinga lokungcola.Ayikho imodi ehleliwe.Isifinyezo sendlela:
Inketho yokuqala:
1. Thela isisombululo sokuhlola ezitsheni ezomile
2. Gcoba sonke isisombululo sokuhlola ku-95% wotshwala, ugeze kabili nge-ultrasonic futhi uwuthulule, ngoba utshwala bungena kalula ku-vial ye-1.5mL futhi bungaxubana nezinyibilikisi eziningi eziphilayo ukuze kuzuzwe umphumela wokuhlanza.
3. Thela emanzini ahlanzekile, futhi ugeze kabili nge-ultrasonically.
4. Thela i-lotion ezitsheni ezomile bese ubhaka ku-110 degrees Celsius amahora angu-1 kuya kwangu-2.Ungalokothi ubhake ezingeni lokushisa eliphezulu.
5. Pholisa futhi wonge.
Inketho yesibili:
1. Hlanza ngamanzi kampompi izikhathi eziningana
2. Yifake kubhekha ogcwele amanzi ahlanzekile (umshini wamanzi ahlanzekile we-Millipore) bese u-sonicate imizuzu engu-15.
3. Shintsha amanzi kanye ne-ultrasound imizuzu engu-15
4. Cwilisa kubhika ogcwele i-ethanol ngokuphelele (Iqembu leSinopharm, I-Analytical Pure)
5. Ekugcineni, yikhiphe futhi uyeke yome.
Inketho yesithathu:
1. Okokuqala cwilisa i-methanol (i-chromatographically pure), futhi ihlanzekile nge-ultrasonically imizuzu engu-20, bese uthela i-methanol eyomile.
2. Gcwalisa izitsha zesampula ngamanzi, futhi uhlanze nge-ultrasonically imizuzu engu-20, uthele amanzi .
3. Yomisa izitsha zesampula ngemva kwalokho.
Inketho yesine:
Indlela yokugeza izitsha zesampula iyafana nokulungiswa kwesigaba se-liquid, njll. Okokuqala, sebenzisa utshwala bezokwelapha ukucwilisa amahora angaphezu kwama-4, bese i-ultrasound isigamu sehora, bese uthela utshwala bezokwelapha, futhi usebenzise amanzi. ingxenye ye-ultrasound.Amahora, hlambulula ngamanzi bese uyomisa.
Inketho yesihlanu:
Okokuqala, cwilisa isisombululo esiqinile sokuhlanza i-oxidizing (i-potassium dichromate) amahora angu-24, bese usebenzisa amanzi angcolile ku-ultrasonic Geza kathathu ngaphansi kwezimo, futhi ekugcineni ugeze nge-methanol kanye, bese uyomisa ukuze usetshenziswe.
Ama-caps septas kufanele ashintshwe, ikakhulukazi uma kuhlaziywa izinsalela zezibulala-zinambuzane, ngaphandle kwalokho kuzothinta imiphumela yobuningi.
Kodwa uma izimo zivuma, zama ukusebenzisa izinto ezilahlwayo, ezifana nezinto ezilahlwayo ze-PTFE noma izinto ezifakwa epulasitiki zasekhaya (cishe yuan/ucezu elingu-0.1), kanye nezitsha zesampula kulungile.Ukusetshenziswa okuphindaphindiwe futhi akudingi ukuhlanzwa.
Inketho yesithupha:
(1) inqubo yokuhlanza eyinkimbinkimbi enemiphumela ephathekayo:
No1.Ngemuva kokuthi kusetshenziswe izitsha zesampula, hlambulula izitsha zesampula ngamanzi agelezayo kuqala, bese uhlanza isampula esele (ungayixhawula ngesandla ngesikhathi esifanayo);
No2, bese ufaka izitsha zesampula kubhamuza eliwuketshezi lokuwasha le-potassium dichromate, futhi lapho linqwabelana Lapho ufinyelela inani elithile noma usesimweni esihle, likhiphe ethangini lokugcoba ulifake kusihlungi sepulasitiki ekhishini. sebenzisa.Hlanza kahle ngamanzi ampompi.Ungakwazi ukuhlunga ngokuphindaphindiwe futhi unyakazise phakathi;
No3.Sebenzisa amanzi kampompi ukuze uhlanze nge-ultrasonically izikhathi ezi-3 ngemva kokugeza.Ngokuzungezile, kungcono ukunyakazisa amanzi ezitsheni zesampula ngemva kokuhlanza ngakunye kwe-ultrasonic;
I-No4, bese usebenzisa amanzi ahlanjululwe kathathu (noma amanzi ahlanzekile, amanzi ahlanjululwe) nge-1.3 yokuhlanza kwe-ultrasonic izikhathi ezintathu;
No5, bese usebenzisa i-chromatographic pure methanol ultrasonic yokuhlanza izikhathi ezingu-2-3, kungcono futhi
nyakazisa i-methanol ezitsheni zesampula ngemva kokuhlanza ngakunye;
No6.Faka izitsha zesampula kuhhavini bese uyomisa cishe ngama-degree angama-80, futhi ingasetshenziswa.
(2) izitsha zesampula ezithengiwe ukuze zimakwe ngemibala ehlukene:
uma uke waqaphela ukuthi kunophawu oluncane olunombala ebhodleleni lesampula, elingelona elokubukeka okuhle, kodwa elinokusetshenziswa kwalo.Lapho uthenga, kungcono ukuthenga izitsha eziningana zemibala ehlukene.
Isibonelo: ilabhorethri yakho ivula amaphrojekthi amabili A no-B ngesikhathi esisodwa.Isikhathi sokuqala iphrojekthi isebenzisa izitsha zesampula ezimhlophe, futhi iphrojekthi engu-B isebenzisa izitsha zesampula eziluhlaza okwesibhakabhaka.Ngemuva kokuthi ukuhlolwa sekuqediwe, kuhlanzwa ngendlela engenhla, futhi ukuhlolwa kwesibili Ngaleso sikhathi, sebenzisa izitsha zesampula eziluhlaza okwesibhakabhaka zephrojekthi A, izitsha zesampula ezimhlophe zephrojekthi ye-B, nokunye, okungagwema ngempumelelo inkinga ebangelwa ukungcola emsebenzini wakho.
Bhala ekugcineni
1. Onjiniyela bezinsimbi abaningana baye basikisela: Sebenzisa isithando somlilo se-muffle at 400 degrees ukubhaka isigamu sehora, izinto eziphilayo zihambile;
2. Faka izitsha zesampula esithandweni se-muffle ukuze zomiswe ku-300 degrees Celsius.Unjiniyela we-Agilent waseBeijing uthe lapho efika esithandweni somlilo, ukuhlolwa ngeke kube nomsindo ngemva kokubhaka esithandweni se-muffle ngama-degree angu-300 amahora angu-6.
futhi ………..
Amaflaski amancane evolumu, amaflaski amise okwepheya okuhwamuka kwe-rotary, nezinye izinto zengilazi zokuhlaziya noma zokwelashwa kwangaphambili zingahlanzwa ngokubhekisela kule ndlela.
Isikhathi sokuthumela: Feb-25-2022